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M94A3278.TXT
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1994-10-25
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Document 3278
DOCN M94A3278
TI Diversity (macrophage-tropism) in the V3 loop region of HIV-1 gp120 from
saliva.
DT 9412
AU Kakizawa J; Ando S; Morishita Y; Nishimura S; Oka S; Mori S; Muller W;
Ushijima H; Department of Microbiology, Institute of Public Health,
Tokyo,; Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):112 (abstract no. PA0068). Unique
Identifier : AIDSLINE ICA10/94369297
AB OBJECTIVE: To analyze the V3 region of HIV-1 gp120 from saliva and to
consider the diversity of HIV-1 from the other organs. MATERIALS AND
METHODS: The saliva and blood samples from HIV positive patients were
collected and HIV DNAs and RNAs were extracted by glass powder. TA
cloning substances of PCR or RT-PCR products with primers of the V3
region were sequenced in several clones in each sample. The results were
compared each other and standard strains. RESULTS: The sequences were
more likely to those from macrophage-tropic cells (SF-162mc strain) than
lymphocyte-tropic cells (MN strain). DISCUSSION: Saliva is easy to use
for clinical diagnosis and for research. The sequence indicates that
saliva would be meaningful to consider the pathogenesis of HIV
infection.
DE Comparative Study Human HIV Envelope Protein gp120/*GENETICS HIV
Infections/MICROBIOLOGY HIV-1/*GENETICS/*ISOLATION & PURIF
Lymphocytes/MICROBIOLOGY Macrophages/MICROBIOLOGY Organ Specificity
Peptide Fragments/*GENETICS Polymerase Chain Reaction
Saliva/*MICROBIOLOGY *Variation (Genetics) MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).